Amy Lyn Acuff (born July 14, 1975, Port Arthur, Texas) is an athlete from the United States. An aggressive high jump competitor, Acuff competed in the 2004 Summer Olympics as a member of USA Track and Field and is a three-time Olympian. Her personal best is 2.01 m, which she achieved in Zürich on 2003-08-15.

Acuff is distantly related to country musician Roy Acuff (her grandfather’s second cousin). (Acuff-Ecoff Family Archives)
- 1993 NCAA Indoor Champion
- 1994 NCAA Indoor Champion
- 1995 NCAA Indoor Champion
- 1995 NCAA Outdoor Champion
- 1995 U.S. Outdoor Champion
- 1996 NCAA Outdoor Champion
- 1997 World University Games Champion
- 1997 NCAA Indoor Champion
- 1997 U.S. Outdoor Champion
- 2001 U.S. Indoor Champion
- 2001 U.S. Outdoor Champion
- 2003 U.S. Outdoor Champion
- 2004 U.S. Indoor Champion

Acuff is also known for her career as a model, including multiple cover appearances:
- Esquire, “Women of Summer: Strength & Beauty: A Portfolio of America’s 10 Sexiest Athletes”
- “Men's magazines”, such as Maxim and FHM
- Playboy, “The Women of the Olympics”